Out of the four nucleobases in DNA3 purine bases have been known to get oxidized at lesser potential. At the other end, when DNA encounters a molecule which causes DNA damage, there is a change in electrochemical signal. These two properties of DNA facilitate the application of DNA in biosensors for the detection of toxins, pollutants and other organic compounds which cause DNA damage. The investigators report a simple two step procedure for the formation of DNA nanostructures (ns-DNA) from a single DNA template sequence of 50nm in size. Obtained nanostructures were electrochemically characterized using differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (E1S) and cyclic voltammetry (CV). For comparison, electrochemical results derived from single stranded (50 bp length) and double stranded (50bp length) DNA based biosensors were used. This obtained ns-DNA when immobilized over modified working electrode surface was observed to act as an efficient biosensor in selective and sensitive identification of aromatic compounds.
展开▼
