A METHOD OF HIGH THROUGHPUT SCREENING OF INHIBITORS THAT INHIBIT INTERACTION OF AN ATTACHMENT PROTEIN TO CELL RECEPTORS USING RECOMBINANT PHAGE DISPLAY

摘要

A METHOD OF IDENTIFYING A POTENTIAL REAGENT TO INHIBIT REACTION BETWEEN TARGET POLYPEPTIDES AND A RECOMBINANT PROTEIN COMPRISES THE STEPS OF PROVIDING A PLURALITY OF TARGET POLYPEPTIDES ATTACHED ON A SOLID PLATFORM; BRINGING A PLURALITY OF A POTENTIAL REAGENT INTO CONTACT WITH AND BIND ONTO THE RECOMBINANT PROTEIN THAT IS EXPRESSED AND DISPLAYED ON PHAGE; BRINGING THE PLURALITY OF THE RECOMBINANT PROTEIN AND POTENTIAL REAGENT INTO CONTACT WITH TARGET POLYPEPTIDES; REMOVING UNBOUND RECOMBINANT PROTEIN AND PHAGE; ADDING A PLURALITY OF BACTERIAL CELLS CAPABLE OF BEING INFECTED BY THE PHAGE TO THE SOLID PLATFORM; AND DETERMINING THE PRESENCE OR NUMBER OF THE INFECTED BACTERIAL CELLS; WHEREBY BINDING OF THE POTENTIAL REAGENT ONTO THE RECOMBINANT PROTEIN INHIBITS BINDING OR INTERACTION BETWEEN THE TARGET POLYPEPTIDES AND THE RECOMBINANT PROTEIN AS WELL AS SUBSEQUENT INFECTION OF THE BACTERIAL CELLS, WHILE INCAPABILITY OF THE POTENTIAL REAGENT TO BIND ONTO THE RECOMBINANT PROTEIN ALLOWS SUBSEQUENT INFECTION OF THE BACTERIAL CELLS BY THE RECOMBINANT PHAGE.