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Method to amplify the pentose phosphate pathway in yeast strains, yeasts obtained and their applications

机译:在酵母菌株中扩增戊糖磷酸途径的方法,获得的酵母及其应用

摘要

Method for obtaining mutant strains of yeast that have an amplified PPV (pentose phosphate pathway), characterized in that it comprises the steps - of culturing the strains on a substrate based on a gluconic acid salt, - of adaptation during at least 10 generations, at an incubation temperature of 16ºC to 32ºC, preferably 28ºC, under shaking, - of selection of the adapted strains that have accumulated mutations that allow an amplification of the PPV, by growth test, on the cultures that they have an OD greater than at least 1.3 times that of the parental strain.
机译:获得具有扩增的PPV(磷酸戊糖途径)的酵母突变菌株的方法,其特征在于,该方法包括以下步骤:-在葡糖酸盐的基础上在基础上培养菌株,-在至少10代中适应。在摇动下,培养温度为16ºC到32ºC,最好是28ºC-选择适应菌株,这些菌株具有积累的突变,允许通过生长测试在其OD值大于1.3的培养物中扩增PPV倍于亲本菌株。

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