Hyperpolarized [U-H-2, U-C-13] Glucose Reports on Glycolytic and Pentose Phosphate Pathway Activity in EL4 Tumors and Glycolytic Activity in Yeast Cells

摘要

Purpose: A resonance at similar to 181 ppm in the C-13 spectra of tumors injected with hyperpolarized [U-H-2, U-C-13] glucose was assigned to 6-phosphogluconate (6PG), as in previous studies in yeast, whereas in breast cancer cells in vitro this resonance was assigned to 3-phosphoglycerate (3PG). These peak assignments were investigated here using measurements of 6PG and 3PG C-13-labeling using liquid chromatography tandem mass spectrometry (LC-MS/MS)