PREPARATION OF ENANTIOPURE VICINAL DIOLS AND ALPHA-HYDROXYKETONES FROM RACEMIC AND MESO-EPOXIDES BY TANDEM BIOCATALYSIS VIA ENANTIOSELECTIVE HYDROLYSIS AND OXIDATIONS

摘要

The invention provides methods of making enantiopure vicinal diols and α-hydroxyketones by biocatalysis with high yield, purity, and substrate scope, e.g., by one-pot biocatalysis. For example, in some embodiments, the invention provides compositions comprising an enantioselective oxidative enzyme and at least one of a selective epoxide hydrolase and a co-factor-regenerating enzyme that can be employed in the methods provided by the invention.