The invention relates to an apparatus, a method and a computer program for investigation of microtiter plates subjected to orbital shaking. The apparatus 10 comprises a housing 7, microtiter plates Ia, Ib, Ic, Id comprising wells, which may be arranged under respective clamp elements 2a, 2b, 2c, 2d arranged to hold the microtiter plate down to the frame 5. The apparatus 10 further comprises a processor 8 conceived to interrupt the motor of the frame 5 for a suitable interrupt period and to trigger the flatbed scanner 3a, 3b for generating two-dimensional images of respective bottoms of the wells present in the microtiter plates 1a, 1b, 1c, 1d. The flatbed scanner 3a, 3b is preferably mounted on respective frames 6a, 6b for support. The frames 6a, 6b may be attached to the housing 7. The processor 8 may be operable using a computer program 8a. The processor may be arranged to store the acquired images in a suitable memory unit 9. Alternatively or additionally, the processor 8 may be arranged to carry-out on-line or off-line analysis of the scan data using calibration data 8b and to adapt shaking mode using a signal S.
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