MODIFICATION OF THE XYLAN UTILIZATION SYSTEM FOR PRODUCTION OF ACIDIC XYLOOLIGOSACCHARIDES FROM LIGNOCELLULOSICS

摘要

Genetically modified microorganisms, that lack or which comprise an inactivated secreted endoxylanase of glycoside hydrolase family (GH) 10 or a homolog thereof, if present within the genome of the microorganism; that lack or comprise an inactivated secreted endoxylanase of GH11 or a homolog thereof, if present within the genome of the microorganism, and/or that lack or comprise an inactivated secreted endoxylanase belonging to GH 30 or a homolog thereof, if present within the genome of the microorganism. A method of producing xylooligosaccharides with or without arabinofuranosyl substitutions (XOS and A-XOS), and/or acidic derivatives thereof (U-XOS and U-AXOS), comprising culturing the microorganisms of the current invention in a culture medium comprising methylglucuronoxylans (MeGX_n) and/or methylglucronoarabinoxylans (MeGAX_n) under conditions that allow conversion of MeGXn and/or MeGAX_n to XOS, AXOS, U-XOS, and/or U-AXOS.