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MODIFICATION OF THE XYLAN UTILIZATION SYSTEM FOR PRODUCTION OF ACIDIC XYLOOLIGOSACCHARIDES FROM LIGNOCELLULOSICS
MODIFICATION OF THE XYLAN UTILIZATION SYSTEM FOR PRODUCTION OF ACIDIC XYLOOLIGOSACCHARIDES FROM LIGNOCELLULOSICS
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机译:从木质纤维素生产酸性木糖寡糖的木聚糖利用系统的改进
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摘要
Genetically modified microorganisms, that lack or which comprise an inactivated secreted endoxylanase of glycoside hydrolase family (GH) 10 or a homolog thereof, if present within the genome of the microorganism; that lack or comprise an inactivated secreted endoxylanase of GH11 or a homolog thereof, if present within the genome of the microorganism, and/or that lack or comprise an inactivated secreted endoxylanase belonging to GH 30 or a homolog thereof, if present within the genome of the microorganism. A method of producing xylooligosaccharides with or without arabinofuranosyl substitutions (XOS and A-XOS), and/or acidic derivatives thereof (U-XOS and U-AXOS), comprising culturing the microorganisms of the current invention in a culture medium comprising methylglucuronoxylans (MeGXn) and/or methylglucronoarabinoxylans (MeGAXn) under conditions that allow conversion of MeGXn and/or MeGAXn to XOS, AXOS, U-XOS, and/or U-AXOS.
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