ELECTRIC MEASUREMENT OF MONOLAYERS FOLLOWING PRO-CLEAVE DETECTION OF PRESENCE AND ACTIVITY OF ENZYMES AND OTHER TARGET ANALYTES

摘要

This invention describes methods for detecting target analytes by utilizing an electroactive moiety (EAM) that functions as a substrate for a specific enzyme. If target analyte is present, a functional group is enzymatically removed from a transition metal complex resulting in quantifiable electrochemical signal at two unique potentials, E01 and E02 that is detected through a self-assembled monolayer (SAM) on an electrode.