Real-time monitoring or diagnostic method for the stem cell senescence by measuring the electric cell-substrate impedance

摘要

The present invention is a method of monitoring the aging of stem cells and stem cells in real time relates to a method for diagnosing aging. Specifically, by having a patterned transparent electrode on a transparent substrate by culturing the cells on a cell chip for generating the response potential of stem cells and culture medium are cultured on the electrode and the total impedance measured from the cultured cells and the culture medium and the electrode analysis and by monitoring the aging, a method for stem cells to target cells and diagnose aging of certain passages can perform the process of comparing the total impedance values measured for each of the age of the subject to diagnose the stem cell relates. With the method of the present invention can be monitored in real time the level of cells to be cultured with non-aging method, can be useful for quality control of the therapeutic agent comprising the cell or an extract of the culture is. ;