Method for Preparing Phosphoenolypruvate carboxylase of Photobacterium profundum SS9 using Escherichia coli system

摘要

The present invention relates to a method for preparing a phosphoenolpyruvate carboxylase of Photobacterium profundum SS9 using an E. coli system, comprising the steps of (a) culturing a phosphoenolpyruvate carboxylase of Photobacterium profundum SS9 Codon optimization of the coding nucleotide sequence for expression in Escherichia coli to obtain the nucleotide sequence of the first sequence of Sequence Listing; (b) inserting a codon-optimized nucleotide sequence of the phosphoenolpyruvate carboxylase into an expression vector to prepare a recombinant vector; And (c) introducing the recombinant vector into E. coli to produce a transformed E. coli.