METHOD FOR PREPARING PHOSPHOENOLYPRUVATE CARBOXYLASE OF PHOTOBACTERIUM PROFUNDUM SS9 USING ESCHERICHIA COLI SYSTEM

摘要

The present invention relates to a manufacturing method of enzyme for phosphoenolypruvate acid carboxylation of photobacterium profundum SS9 using an E. coli system comprising the steps of: (a) obtaining a first nucleotide sequence on a sequence list by codon-optimizing a nucleotide sequence, coding the enzyme for phosphoenolypruvate acid carboxylation of photobacterium profundum SS9, which is suitable for being expressed under E. coli; (b) manufacturing a recombinant vector by inserting the codon-optimized nucleotide sequence of the enzyme for phosphoenolypruvate acid carboxylation into expression vectors; and (c) manufacturing transgenic E.coli by inserting the recombinant vector into E.coli.