Microspectral method of estimating effective pharmacotherapy In TREATMENT EARLY BIRDS KLEBSIELLEZA Antibacterials

摘要

1. microspectral method for evaluating the effectiveness of drug therapy in the early stages of treatment klebsielleza birds antibacterial drugs, the method comprising the double-wavelength luminescent spectral analysis in the study of histological sections stained with specific fluorescent dye-labeled dichloro-s-triazinilaminofluorestseinom-1 and ethidium bromide, wherein the performed study histological sections of tissue glandular stomach of healthy controls of poultry and birds surveyed at each histological th slice using visual microscopy define an area of ​​epithelium of deep alveolar glands mucosa, in which there are five regions with the highest degree of luminescence intensity from each plot was prepared luminescence spectrum and the spectrum of the optical density and spend their digital processing: a luminescence spectrum recorded value band luminescence intensity radiation at a wavelength characteristic of the epithelial deep alveolar zone proteins mucosal glands and equal to 528 nm and at wavelength characteristic of nucleic acids epithelium zone deep alveolar gland mucous membrane and equal to 624 nm in the spectrum of the optical density of the recorded value of the optical density at a wavelength of 648 nm, which is used as the photometric portion thickness, and then determine the amount of proteins and nucleic acids in each portion in the conventional units with respect to the value of the obtained values ​​of optical density to the standard, which is used as the magnitude of the luminescence intensity at the wavelength of uranium glass