MICROSPECTRAL METHOD FOR ASSESSING EFFICACY OF PHARMACOTHERAPY ACCOMPANYING EARLY TREATMENT OF AVIAN KLEBSIELLA WITH ANTIBACTERIAL PREPARATIONS

摘要

FIELD: medicine.;SUBSTANCE: invention refers to veterinary science and can be used for assessing the efficacy of pharmacotherapy for the first 7 days of treating avian Klebsiella with antibacterial preparations. A method of two-wave luminescent spectral analysis is used to study histological sections of avian fore-stomach tissue coloured with specific luminescent stain labels, dichlor-symm-triazinylaminofluorescein-1 and ethidium bromide. That is combined with studying the histological sections of the fore-stomach tissue of healthy and analysed birds. An epithelial area of deep alveolar glands of the mucous membrane is localised on each histological section, and five segments with the greatest luminescence intensity are selected in the above area. A luminescence spectrum and an optical density spectrum of each segment is derived They are digitally processed: luminescence intensity is recorded in the luminescence spectrum at a wave length of 528 nm specific for proteins and at a wave length of 624 nm specific for nucleic acids. Optical density is recorded in the optical density spectrum at a wave length of 648 nm, which is used as a photometric area thickness; then an amount of proteins is determined in each segment by formula $B=\frac{I_b}{D\times E},\mathop{\mathrm{image}}$ and an amount of nucleic acids is determined by formula $N=\frac{I_n}{D\times E},\mathop{\mathrm{image}}$ wherein B is the amount of proteins in standard units, in each of the five segments of the epithelium, Ib is the luminescence intensity of the analysed epithelium at a wave length of 528 nm, N is the amount of nucleic acids in standard units in each of the five segments of the epithelium, In is the luminescence intensity of the analysed area, D is the optical density of the photometric area; E is the luminescence intensity of uranium glass. An average amount of proteins and nucleic acids is calculated in standard units. Indicators of an intracellular metabolism of proteins and nucleic acids in the fore-stomach of the analysed bird are calculated. If the indicator of the intracellular metabolism of proteins is more than 0.95, and if the indicator of the intracellular metabolism of nucleic acids is more than 0.87, the low efficacy of the pharmacotherapy is stated. If the indicators of the intracellular metabolism of proteins are 0.95-0.25, and those of nucleic acids are 0.87-0.21, the moderate efficacy of the pharmacotherapy is stated. If the indicators of the intracellular metabolism of proteins are less than 0.25, and those of nucleic acids are less than 0.21, the high efficacy of the pharmacotherapy in the analysed bird is stated.;EFFECT: method enables providing the more accurate diagnosis of the intracellular metabolism of proteins and nucleic acids in the avian fore-stomach.;2 tbl, 2 dwg