METHOD FOR DETECTING VAGINITIS IN FEMALES OF REPRODUCTIVE AGE BY EXPRESSION LEVEL OF mRNA OF CYTOKINE GENES IN VAGINAL SMEARS

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine and aims at diagnosing vaginitis in females of a reproductive age. Expression levels of mRNA of TNF, IL18, GATA3 and TLR4 genes are measured in vaginal smears. Expression levels of mRNA of TLR4/GATA3 and TNF/IL18 genes are related, and the derived threshold cycles are used to predict a probability of vaginitis by formula. If P≤57% for nonpregnant females or P≤68.5% for pregnant females, the absence of vaginitis is stated. If P≥57% for nonpregnant females or P≥68.5% for pregnant females, vaginitis is diagnosed.;EFFECT: invention provides the effective diagnosis of vaginitis in the females of a reproductive age.;3 tbl, 5 ex