FLAMMENIONIZATION DETECTION FOR OVERCRITICAL FLUID CHROMATOGRAPHY USING A FIXED COLUMN AND A FILM FLAME ADJUSTED

摘要

The present invention relates to flame-based detection methods for compressed mobile phase chromatography. In particular, the present invention relates to the operation of a flame ionization detector for carbon dioxide based chromatography, such as supercritical fluid chromatography. The present invention includes a method for fitting a chromatographic column to an internal burner of the flame ionization detector, including providing a chromatographic column having an inside diameter, determining an optimum flow rate of the mobile phase for the chromatographic column, calculating an optimum inside diameter of the internal burner along with the inside diameter and the flow rate of the column gives optimum detector performance; and providing an internal burner of the flame ionization detector having an inside diameter substantially equal to the calculated optimum inside diameter.