Novel phytase, method for obtaining the same and use thereof

摘要

The invention generally relates to novel and improved nucleic acid sequences encoding a polypeptide or protein having the enzymatic activity of a phytase and methods for effectively expressing such enzymes in a host cell.;In a first aspect, the present invention relates an isolated nucleic acid molecule i) comprising the nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity or ii) having a sequence identity of at least 78 % to a nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity.;The present invention also relates to an expression construct or a vector comprising the above mentioned isolated nucleic acid molecule. The present invention further relates to a method of producing a protein having the enzymatic activity of a phytase comprising the steps of:a) introducing into a host cell a vector comprising:i) elements regulating the expression that are functional in the host cell; andii) operatively linked thereto a nucleic acid molecule according to the invention;b) cultivating the host cells obtained in step a) under conditions suitable for expression of the protein, and optionallyc) recovering the protein produced in step b) from the cell culture.;The present invention further relates to proteins obtained by the method according to the invention and the use thereof for the manufacture of additives for animal feed.