Assay methods utilizing surface plasmon excitation enhanced fluorescence spectroscopy

摘要

Relates metering condition setting step is an assay method specific process using the SPFS, to improve the effects in the use of whole blood samples, in particular, blood cells remaining in the measuring solution at the time of SPFS measurement doing, the absolute value suppresses to fluctuations in the background signal, called a resonance angle and optical blank, provides an assay method utilizing surface plasmon excitation enhanced fluorescence spectroscopy. A primary reaction step of the sample, a first cleaning step, the labeling reaction step, an assay method utilizing the surface plasmon excitation enhanced fluorescence spectroscopy and a measurement process, wherein the first reaction step of the labeling reaction step during, the assay method utilizing the surface plasmon excitation enhanced fluorescence spectroscopy, including the evaluation step of the blood cell component amount, to solve the above problems