Assay method using a sensor chip for a fluorescence measuring device using surface plasmon excitation enhanced fluorescence spectroscopy, and assay kit

摘要

An assay method with use of a sensor chip which includes a metal member, a self-assembled monolayer (SAM), and ligands on a support, and is configured to be used fur a fluorescence measuring apparatus with utilization of a surface plasmon-field enhanced Fluorescence Spectrometry, including the steps of: forming a hydrophilic high molecule layer on the self-assembled monolayer in the sensor chip; immobilizing the ligands at least one of in the hydrophilic high molecule layer and on the surface of the hydrophilic high molecule layer; and bringing a moisturizer in contact with the hydrophilic high molecule layer.