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CONSTRUCTS AND METHODS FOR GENOME EDITING AND GENETIC ENGINEERING OF FUNGI AND PROTISTS
CONSTRUCTS AND METHODS FOR GENOME EDITING AND GENETIC ENGINEERING OF FUNGI AND PROTISTS
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机译:真菌和保护基因的基因编辑和基因工程的结构和方法
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摘要
Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.
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