An improved isolator for collection, parametrical characterization and long-term preservation of samples containing stem cells comprises: a work chamber (2) isolated from the external environment; gloves jutting out at an inside of said work chamber (2); an inlet chamber (4) for introduction of a sample to be processed; an outlet chamber connected with the work chamber (2) and having a sterile sample-collecting bag; sterilizing means acting on said sample and/or on said inside of the work chamber (2); filtering means; cooling means for bringing and keeping said sample at a constant working temperature throughout the inlet chamber (4), the work chamber (2) and the outlet chamber; and visual imaging means (5) focused on the sample and defining a visual path which maintains a complete mechanical and pneumatic/hydraulic isolation between said inside of the work chamber (2) and the external environment.
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