Calcium-binding photoproteins showing the luminescence pattern with a slow decay of are desired. The invention provides a mutant apoprotein comprising an amino acid sequence wherein the 23rd to 34th amino acids in the amino acid sequence of SEQ ID NO: 2 are substituted with an amino acid represented by formula I below: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34; having a function to bind to the peroxide of coelenterazine or the peroxide of a coelenterazine analog to form a photoprotein capable of emitting light under the action of calcium ions; and, having a half decay time of the luminescence emitted by binding of the photoprotein to calcium ions being not less than 2 seconds.
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机译:需要具有缓慢衰变的发光模式的钙结合光蛋白。本发明提供了一种突变脱辅基蛋白,其包含氨基酸序列,其中SEQ ID NO:2的氨基酸序列中的第23至34个氨基酸被下式I表示的氨基酸取代:Xaa23-Xaa24-Xaa25-Xaa26-Xaa27 -Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34;具有与腔肠素过氧化物或腔肠素类似物的过氧化物结合以形成能够在钙离子的作用下发光的光蛋白的功能;并且,通过光蛋白与钙离子的结合而发出的发光的衰变时间为2秒以上。
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