Method for amplification of nucleic acid target sequence or signal, oligonucleotideo modified reversible, mixture and set of nucleic acid amplification reaction.

摘要

Method for amplification of nucleic acid sequence or signal the target oligonucleotide modified peversu00edvel, set of reaction mixture and amplification of nucleic acid.The present invention provides a method for the amplification of a target nucleic acid sequ00fau00eancia or signs, where a mixture of amplification reaction is used containing at least one oligonucleotideo modified reversibly in a tip 3 39 a hydroxyl group.That can be converted into an extremity 3 'hydroxyl following exposure to a chemical and / or irradiation and / or temperature range. This invention also provides a oligonucleotideo reversivelmentemodificado, as described above, and a mixture of reaction for amplification of nucleic acids and a set comprising such oligonucleotide.