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Procedure for Quantitative Monitoring of Endospores in the Aquatic Environment of a Paper or Cardboard Factory
Procedure for Quantitative Monitoring of Endospores in the Aquatic Environment of a Paper or Cardboard Factory
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机译:纸或纸板工厂水生环境中内生孢子的定量监测程序
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摘要
The invention relates to a method for quantitative monitoring of bacterial endospores in an aqueous environment of a paper or board mill. The method comprises at least the following steps: obtaining at least a first aqueous sample originating from the industrial aqueous environment; destroying bacteria in vegetative form in the first sample by a suitable treatment, preferably by heating the first sample to a desired temperature; adding intercalating agent (such as PMA) to the treated first sample and allowing it to interact (e.g. by cross-linking) with the destroyed bacteria, so that the nucleic acid from the destroyed bacteria are unavailable for PCR; and determining the endospore level in the first sample by using quantitative polymerase chain reaction (qPCR) in which only the DNA from the endospores is available for amplification.
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