METHOD FOR QUANTITATIVE MONITORING OF ENDOSPORES IN WATER ENVIRONMENT OF PAPER OR CARDBOARD FACTORIES

摘要

FIELD: biotechnology.;SUBSTANCE: invention relates to the biotechnology. Disclosed is a method for quantitative monitoring of bacterial endospores in the aqueous medium of paper and cardboard factories. Method includes extraction of two water samples of industrial water medium; in the first sample the vegetative forms of bacteria are destroyed by heating of not less than 60 °C, adding an intercalating agent, incubating in dark for 1–30 minutes, determining endospore level using a quantitative polymerase chain reaction. Level of endospores is compared from the first sample with the number of vegetative bacterial cells in the second sample. Results are used to correct the biocides introduction regimen for endospore control.;EFFECT: method provides monitoring of microbiological state of processes in paper or cardboard production.;10 cl, 3 tbl, 3 ex