DETECTION OF INDIVIDUAL T-CELL REACTION PATTERNS AGAINST TUMOUR-ASSOCIATED ANTIGENS (TAAS) IN TUMOUR PATIENTS AS A BASIS FOR THE INDIVIDUALIZED THERAPEUTIC VACCINATION OF PATIENTS

摘要

The present invention relates to a method for identifying the preferentialtarget antigens ofantitumoural T-cells of a tumour patient, comprising: a) providing T-cellsfrom the blood of atleast one tumour patient, b) providing dendritic cell (DCs) and/or B-lymphocytes (BLCs) that areautologous for said tumour patient, wherein said DCs and BLCs were transfectedbeforehandwith a selection of mRNAs encoding for T-cell-immunogenic tumour-associatedantigens (TAA),and express these, c) contacting said T-cells with the DCs and/or BLCs, d)identifying of thoseT-cells that recognize antigens of the DCs and /or BLCs, and e) identifying ofthe preferential targetantigens of antitumoural T-cells of the at least one tumour patient on thebasis of the T-cells thatrecognize antigens of the DCs and /or BLCs. The method can furthermorecomprise theexpansion of the T-cells that recognize the antigens of the DCs and/or BLCs.The presentinvention furthermore relates to a method for producing an individualizedtumour vaccine orindividualized tumour therapeutic, as well as corresponding methods fortreating a tumourousdisease using the individualised tumour vaccine or individualised tumourtherapeutic.