Assay for detecting and quantifying HIV-1

摘要

#$%^&*AU2014201154B220160211.pdf#####ASSAY FOR DETECTING AND QUANTIFYING HIV-1 ABSTRACT Compositions, methods and kits for detecting HIV- 1 nucleic acids using nucleic acid amplification. Particularly described are oligonucleotides that are useful as hybridization probes 5 and amplification primers for detecting very low levels of HIV- 1 nucleic acids by real-time monitoring of amplicon production. The invented assays are characterized by high levels of precision in the quantitation of HIV- 1 targets at low copy numbers, and by accurate detection of different HIV-1 subtypes, including M group and 0 group variants.