A NOVEL HIGH-THROUGHPUT METHOD FOR QUANTIFICATION OF HBV CCCDNA FROM CELL LYSATE BY REAL-TIME PCR

摘要

The present invention provides (1) a set of novel DNA primers that can be used for high efficient real-time qPCR of Hepatitis B virus (HBV) circular DNA (cccDNA) and (2) a novel high throughput method for quantification of HBV cccDNA from cells using this set of novel DNA Primers by real-time qPCR. It also provides a use of this method in monitoring cccDNA level in experimentally infected liver cells and evaluation of therapeutic effect on HBV.