METHOD FOR PRODUCING VACCINE AND MEASURING ANTIBODY, USING OUTER SKIN PROTEIN OF PORCINE EPIDEMIC DIARRHEA VIRUS

摘要

The present invention relates to a method for producing a vaccine and measuring an antibody with respect to the virus, using an outer skin protein of a porcine epidemic diarrhea virus and, more particularly, to a method for infecting a Vero cell with an outdoor isolate of PEDV, and separating and extracting the outer skin protein of a PED virus therefrom; a method for producing a vaccine of a PED virus produced by uniformly mixing the outer skin protein of the separated PED virus with an immune booster including mineral oil and an emulsifier or a suspension promoter; a method for measuring a blood antibody with respect to a PED virus for measuring a concentration of an antibody by a RIDEA method in which ELISA and the principle of radioimmunoassay diffusion are combined by absorbing the outer skin protein (antigen) of the separated PED virus in a substrate to be reacted with a pig serum; and a method for measuring an antibody a cell with respect to a PED virus measured in a delayed type hypersensitivity reaction (DTH) method by intradermally inoculating the outer skin protein of the separated PED virus to a testing pig. According to the present invention, the method for producing a vaccine and a method for measuring an antibody, using the outer skin protein of a porcine epidemic diarrhea virus is capable of preventing mortality of newborn piglets and economically and easily diagnosing whether of infection of a virus of a pig in which infection of a PED virus is suspicious.;COPYRIGHT KIPO 2016