Rapid isolation method for preparation of immuno-stimulating polysaccharides from persimmon leaves

摘要

The present invention relates to a method for rapidly separating polysaccharides having immunity-enhancing activity from persimmon leaves. More specifically, the present invention relates to a method for rapidly separating polysaccharides from persimmon leaves, comprising: (a) decomposing persimmon leaves with a pectin cleaving enzyme and removing solids; (b) precipitating the hydrolyzate of step (a) with 70% (v / v) to 95% (v / v) ethanol to separate and obtain the precipitate; (c) recovering the precipitate of step (b) and precipitating with 45% (v / v) to 55% (v / v) ethanol to separate and obtain the supernatant and the precipitate; (d) recovering the precipitate from step (c) and precipitating the precipitate with 40% (v / v) to 45% (v / v) ethanol to separate and obtain the supernatant and the precipitate; (e) recovering the supernatant of step (c) and the supernatant of step (d) to obtain rhamogalactoranol 2; (f) recovering the precipitate from step (d) to obtain rhamogalactoranyl 1, and a method for producing high immunosuppressive polysaccharide derived from persimmon leaves, and high-immuno-active polysaccharide prepared from the persimmon leaves . The method for producing high-immuno-active polysaccharide derived from persimmon leaves of the present invention is a method for separating and purifying a specific polysaccharide from a polysaccharide mixture more rapidly and efficiently than a conventional method for isolating and purifying a polysaccharide mixture Excellent effect.