1 METHOD FOR DETECTING A PLURALITY OF NUCLEOTIDE POLYMORPHISMS AT A SINGLE WAVELENGTH USING A PLURALITY OF OLIGONUCLEOTIDES MODIFIED WITH FLUORESCENT DYE HAVING THE SAME OR CLOSE DETECTION WAVELENGTH

摘要

[Problem] The present invention, if there is a restriction on the type of the fluorescence wavelength to the measuring device is detected, or, even if there is a restriction on the types of fluorescent molecules that can be modified in the probe, at a time, a number of different mutations to be a challenge to provide a method of measurement. And labeled with [MEANS FOR SOLVING PROBLEMS] fluorescent dye, an oligonucleotide that hybridizes to the region containing the gene polymorphism as a method by multi-use to detect a plurality of genetic polymorphisms at the same time, a fluorescent dye for labeling a plurality of oligonucleotides with each other and the same or a fluorescent dye having a detection wavelength in the vicinity of, the plurality of oligonucleotides, Tm value for a sequence comprising the first genotype of the plurality of gene polymorphism is designed such that the temperature in the vicinity of each other, It said plurality of oligonucleotides comprises a plurality of genetic polymorphisms in each, and the Tm value of the sequence comprising a second genotype and Tm value of the sequence comprising the first polymorphism is designed to fall more than 3 ℃, one kind of wavelength the method characterized in that at the same time detected. ;