A single nucleotide polymorphism in the matrix metalloproteinase 2 promoter is closely associated with high risk of nasopharyngeal carcinoma in Cantonese from southern China

摘要

Matrix metalloproteinase 2 (MMP2) has been shown to play an important role in several steps of cancer development.The-1306C/T polymorphism of the MMP2 gene displays a strikingly lower promoter activity than the T allele,and the CC genotype in the MMP2 promoter has been reported to associate with the development of several cancers.To assess the contribution of the MMP2-1306c/r polymorphism to the risk of nasopharyngeal carcinoma (NPC),we conducted a case-control study and analyzed MMP2 genotypes in 370 patients with NPC and 390 frequency-matched controls using real-time PCR-based TaqMan allele analysis.We found that subjects with the CC genotype had an increased risk (OR = 1.55,95% Cl = 1.05-2.27) of developing NPC compared to those with the CT or TT genotypes.Furthermore,we found that the risk of NPC was markedly increased in subjects who were smokers (OR = 15.04,95% Cl = 6.65-33.99),heavy smokers who smoked ≥20 pack-years (OR = 18.66,95% Cl = 7.67-45.38),or young (＜60 years) at diagnosis (OR = 1.52,95% Cl = 1.01-2.29).Our results provide molecular epidemiological evidence that the MMP2-1306c/r promoter polymorphism is associated with NPC risk,and this association is especially noteworthy in heavy smokers.