Method for detecting false negative amplification of nucleic acids using polynucleotide comprising deoxyuridine as indicator for UDG activity

摘要

present application discloses a polynucleotide, a nucleic acid amplification reaction of the composition for preventing contamination and pollution prevention method for nucleic acid amplification reaction comprising them that include one or more zero-dUMP labeled fluorescent dyes and fluorescence cancellation. Polynucleotides and methods according to the present application can use the fluorescence detection apparatus for activity of UDG can be quickly and easily monitored in real-time whether the UDG enzyme activity, to avoid false positives that may occur in the nucleic acid detection, thereby increasing the reliability of the results . ;