首页> 外国专利> Probe set for diagnosing or detecting Cowpea chlorotic mottle virus and uses thereof

Probe set for diagnosing or detecting Cowpea chlorotic mottle virus and uses thereof

机译:诊断或检测Cow豆绿斑驳斑病毒的探针组及其用途

摘要

The invention provides a Cowpea chlorotic mottle virus (CCMV) oligonucleotide comprising at least one oligonucleotide selected from the group consisting of an oligonucleotide of SEQ ID NO: 371, SEQ ID NO: 416 to 419 and SEQ ID NO: 436 to 443 and complementary oligonucleotides thereof, A probe set, a CCMV diagnostic or detection gene chip containing the oligonucleotide probe set, separating RNA from a sample presumed to be infected with CCMV, synthesizing cDNA using the separated RNA as a template, A method for diagnosing or detecting CCMV comprising contacting a cDNA with a set of oligonucleotide probes to hybridize the cDNA with a probe set and detecting the degree of hybridization between the probe and the cDNA and a method for diagnosing or detecting the CCMV using the oligonucleotide probe set and hybridization solution It relates to a kit comprising CCMV.
机译:本发明提供了pea豆绿斑驳病毒(CCMV)寡核苷酸,其包含至少一种选自SEQ ID NO:371,SEQ ID NO:416至419和SEQ ID NO:436至443的寡核苷酸和互补的寡核苷酸一种探针组,一种包含寡核苷酸探针组的CCMV诊断或检测基因芯片,从假定感染了CCMV的样品中分离RNA,使用分离的RNA作为模板合成cDNA,一种用于诊断或检测CCMV的方法,包括接触一种具有一组寡核苷酸探针的cDNA,以使该cDNA与探针组杂交并检测该探针与cDNA之间的杂交程度,以及使用该寡核苷酸探针组和杂交溶液的诊断或检测CCMV的方法,涉及一种试剂盒包括CCMV。

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