Oct4 Sox2 A method for reprogramming of human dental pulp cell using Oct4 and Sox2 and use thereof

摘要

The present invention relates to a method for producing induced pluripotent stem cells using only the reprogramming factors Oct4 and Sox2 in human dental caries cells and a method for differentiating induced pluripotent stem cells produced by the above method into vascular progenitor cells . The present invention also relates to a method for differentiating vascular endothelial cells, comprising the step of differentiating the differentiated vascular progenitor cells into vascular endothelial cells through the above-mentioned vascular progenitor cell differentiation method. In addition, the present invention relates to a method of differentiating smooth muscle cells, comprising the step of differentiating the differentiated vascular progenitor cells into smooth muscle cells through the above differentiation method of vascular progenitor cells.In addition, the present invention relates to an inducible pluripotent stem cell having improved vascular progenitor cell differentiation prepared by the method for producing an induced pluripotent stem cell, a vascular endothelial cell prepared by the vascular progenitor cell differentiation method, Progenitor cells. In addition, the present invention relates to vascular endothelial cells prepared by the vascular endothelial cell differentiation method or smooth muscle cells prepared by the smooth muscle cell differentiation method.The present invention also relates to a pharmaceutical composition for treating ischemic diseases comprising at least one selected from the group consisting of the above-mentioned induced pluripotent stem cells, vascular progenitor cells, vascular endothelial cells and smooth muscle cells as an active ingredient. In particular, the above therapeutic composition relates to a pharmaceutical composition for treating lower limb ischemia or myocardial infarction in ischemic diseases.The present invention provides a method for efficiently producing pluripotent stem cells derived from human dental caries cells using only two dedifferentiation factors, Oct4 and Sox2, which are non-tumorigenic genes. According to the present invention, as compared with existing tissue cell resources, the patient's dermal cells are excellent cell resources for producing induced pluripotent stem cells with high efficiency. In addition, the present invention provides an optimized method for differentiating into a vascular precursor cell and inducing differentiation into a vascular endothelial cell and a smooth muscle cell from a stem cell-derived induced pluripotent stem cell of a patient. In addition, the present invention is expected to contribute to the treatment of ischemic diseases since the differentiated cells derived from the patient's dermal gut cells can be used as a cell therapy agent by examining the cell therapy effect of the vascular progenitor cells in the lower limb ischemic and myocardial infarction model.;