ANTIGENS WITH A WIDE REACTIVITY SPECTRUM OPTIMIZED BY COMPUTER MEANS FOR INFLUENZA H5N1 AND H1N1 VIRUSES

摘要

1. Recombinant hemagglutinin (HA) polypeptide of influenza virus containing the amino acid sequence of residues 2-566 of SEQ ID NO: 2 or residues 2-568 of SEQ ID NO: 1.2. The influenza virus HA polypeptide according to claim 1, consisting of the amino acid sequence of residues 2-566 of SEQ ID NO: 2 or residues 2-568 of SEQ ID NO: 1.3. The influenza virus HA polypeptide according to claim 1, comprising the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 1.4. The influenza virus HA polypeptide according to claim 1, consisting of the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 1.5. An isolated nucleic acid molecule encoding an influenza virus HA polypeptide according to any one of claims 1 to 4. The isolated nucleic acid molecule according to claim 5, wherein the nucleic acid molecule is subjected to codon optimization for expression in mammalian cells. A vector containing a nucleic acid molecule according to claim 5 or 6.8. The vector of claim 7, further comprising a promoter operably linked to a nucleic acid sequence encoding an influenza virus HA polypeptide. An isolated cell containing the vector according to claim 7 or 8.10. A virus-like particle (VLP) similar to a particle of an influenza virus, comprising an influenza virus HA polypeptide according to any one of claims 1 to 4.11. A VLP similar to the influenza virus particle of claim 10, further comprising an influenza virus neuraminidase (NA) protein, an influenza virus matrix protein (M1), or both. A VLP similar to a particle of an influenza virus, comprising an influenza virus HA polypeptide according to any one of claims 1 to 4, obtained by transfecting a host cell with a vector encoding an HA polypeptide, a vector encoding an influenza virus NA protein, and a vector encoding an M1 protein influenza virus, under conditions sufficient to ensure the expression of HA, M1 and NA.13 proteins. A fusion protein containing an influenza virus HA polypeptide according to any one of claims 1 to 4.14. Composition containing influenza HA polypeptide