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APPLICATION OF A HEATING DEVICE FOR ACTIVATION OF A BIOLOGICAL REACTION

机译:加热装置在生物反应活化中的应用

摘要

1. The method of conducting a biological, biochemical or chemical reaction requiring incubation at a temperature of from 30 to 40 ° C, characterized in that it includes the step of activating a heating device, the action of which is based on an exothermic physical or chemical process, and the step of bringing said heating device into contact with the container containing the reagents involved in this reaction. 2. The method according to claim 1, characterized in that the step of bringing the heating device into contact with the container is carried out by placing the heating device and at least the lower part of the container in the cavity of the tripod intended for this purpose. A method according to claim 1 or 2, characterized in that the container contains an enzymatic solution and that the contact between the heating device and the container is maintained for at least 10 minutes. The method according to claim 1, characterized in that the heating device consists of a sealed plastic bag containing a saturated aqueous solution of sodium acetate. The method according to claim 4, characterized in that the volume of the solution containing the reactants involved in the reaction is from 3 to 10 ml, the container containing the specified solution is a Petri dish having a diameter of from 7 to 11 cm, or the chamber of the specified cup , and the fact that the heating device is a disk, the thickness of which is from 3 to 7 mm and the diameter is from 7 to 11 cm. 6. The method according to p. 2 and 5, characterized in that the cavity of the tripod, designed to accommodate the heating device, is round, with a diameter of from 7 to 11.5 cm, and has a depth of from 5 to 20 mm. The method of claim 1 for separating cells originating from a tissue sample, comprising the following steps: (i) initiation
机译:1.进行需要在30至40℃的温度下孵育的生物,生化或化学反应的方法,其特征在于,该方法包括激活加热装置的步骤,该加热装置的作用基于放热的物理或化学反应。化学过程,以及使所述加热装置与装有该反应所涉及的试剂的容器接触的步骤。 2.根据权利要求1所述的方法,其特征在于,使所述加热装置与所述容器接触的步骤是通过将所述加热装置和所述容器的至少下部放置在用于该目的的三脚架的空腔中来进行的。目的。 3.根据权利要求1或2所述的方法,其特征在于,所述容器容纳酶溶液,并且所述加热装置与所述容器之间的接触保持至少10分钟。 2.根据权利要求1所述的方法,其特征在于,所述加热装置由密封的塑料袋组成,所述塑料袋容纳有乙酸钠的饱和水溶液。 5.根据权利要求4所述的方法,其特征在于,所述反应中所含反应物的溶液体积为3至10ml,所述特定溶液的容器为培养皿,其直径为7-11cm,或指定杯子的腔室,加热装置是一个圆盘,其厚度为3到7毫米,直径为7到11厘米。 6.根据p的方法。如图2和5所示,其特征在于,设计成容纳加热装置的三脚架的腔是圆形的,直径为7-11.5cm,深度为5-20mm。 3.根据权利要求1所述的用于分离源自组织样本的细胞的方法,包括以下步骤:(i)起始

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