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APPLICATION OF A HEATING DEVICE FOR ACTIVATION OF A BIOLOGICAL REACTION
APPLICATION OF A HEATING DEVICE FOR ACTIVATION OF A BIOLOGICAL REACTION
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机译:加热装置在生物反应活化中的应用
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摘要
1. The method of conducting a biological, biochemical or chemical reaction requiring incubation at a temperature of from 30 to 40 ° C, characterized in that it includes the step of activating a heating device, the action of which is based on an exothermic physical or chemical process, and the step of bringing said heating device into contact with the container containing the reagents involved in this reaction. 2. The method according to claim 1, characterized in that the step of bringing the heating device into contact with the container is carried out by placing the heating device and at least the lower part of the container in the cavity of the tripod intended for this purpose. A method according to claim 1 or 2, characterized in that the container contains an enzymatic solution and that the contact between the heating device and the container is maintained for at least 10 minutes. The method according to claim 1, characterized in that the heating device consists of a sealed plastic bag containing a saturated aqueous solution of sodium acetate. The method according to claim 4, characterized in that the volume of the solution containing the reactants involved in the reaction is from 3 to 10 ml, the container containing the specified solution is a Petri dish having a diameter of from 7 to 11 cm, or the chamber of the specified cup , and the fact that the heating device is a disk, the thickness of which is from 3 to 7 mm and the diameter is from 7 to 11 cm. 6. The method according to p. 2 and 5, characterized in that the cavity of the tripod, designed to accommodate the heating device, is round, with a diameter of from 7 to 11.5 cm, and has a depth of from 5 to 20 mm. The method of claim 1 for separating cells originating from a tissue sample, comprising the following steps: (i) initiation
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