METHOD OF ANALYSING CYTOCHROME C IN INTACT MITOCHONDRIA BY GIANT COMBINED DIFFUSION SPECTROSCOPY ON NANOSTRUCTURED COATINGS

摘要

FIELD: bio-analytical examination.SUBSTANCE: present invention relates to bio-analytical research and represents method of analysing cytochrome C in intact mitochondria by giant combined scattering spectroscopy (GCD), involving preparation of mitochondria and their application on substrate based on dielectric chemically inert material with nano-structured coating with thickness of 1-10 mcm in the of ring silver nano-structures rims silver rings consist of communicating with each other porous silver aggregates on the surface of which are rounded silver nano-particles with size of 2-90 nm, with subsequent immobilisation of mitochondria data nano-structured coating GCD spectra detection with subsequent decoding characteristic oscillations of analysed sample GCD spectra using standard software.EFFECT: invention implementation enables extending the range of application GCD and research in intact functioning mitochondria.12 cl, 4 ex, 1 dwg