Method and apparatus for localization of individual dyestuff molecules in the fluorescence microscopy

摘要

The invention relates to a method and apparatus for localization of individual dyestuff molecules in the fluorescence microscopy. The method comprises:Obtaining illumination methods - information of the fluorescently labeled sample to be examined by means of fluorescence far field of the microcapsules a copy, in the use of structured illumination light, wherein the illumination methods - information the spatial extent of the sample in at least one spatial direction and / or the dye in this direction, which are difficult to point position comprise;Location information obtained from the sample by means of a localization of the microcapsules a copy by illuminating the sample with the illumination light with an intensity and / or intensity distribution of which, in an intensity window of approximately 1 kw / cm² to 1 mw / cm² , the fluorescent molecules of the at least one fluorescent marking statistically and individually from a non-fluorescent state to a fluorescent state and with the emission of fluorescence light is converted into a long-life inactive state; andDetecting at least a part of the fluorescent light information obtained by a sensor, as a result of which at least one image of the sample is obtained, and performing a localization of the individual fluorescent molecules in the image space; andCombining the illumination methods - information with the location information.