SENSITIVE DIAGNOSTIC ASSAY FOR INCLUSION BODY MYOSITIS

摘要

Assay methods, kits and systems for an improved sensitive diagnosis of inclusion body myositis (IBM) are provided. Assays, kits and systems involve detecting the presence and/or level of a combination of more than one autoantibody isotypes that are reactive against a cytosolic 5-nucleotidase 1A protein (CN1A), or a cytosolic 5-nucleotidase 1B protein (CN1B), or a CN1B isoform thereof, or a peptide fragment thereof, an isolated peptide thereof or a fusion protein comprising an isolated peptide of CN1A or CN1B. IBM patients have differing patterns of anti-cN1A/cN1B autoantibody isotypes. Therefore, testing a combination of such isotypes provide an improved and sensitive diagnostic assay.