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Method for producing lactone from a strain of Aureobasidium pullulans (AUREOBASIDIUM PULLULANS)
Method for producing lactone from a strain of Aureobasidium pullulans (AUREOBASIDIUM PULLULANS)
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机译:从金黄色葡萄球菌(Aureobabasidium Pullulans)菌株生产内酯的方法
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摘要
The present invention relates to a method for producing a lactone from a strain of Aureobasidium pullulans. The present invention also relates to a method wherein the method comprises the following steps: before the step of Aureobasidium pullulans strain CBS 771.97 (obtained from CBS-KNAW Fungal Biodiversity Center, Uppsalalaan 8, 3584 CT Utrecht, Netherlands) A step of culturing, Cultures are produced from the inoculum obtained from the preculture by fermentation at a temperature of 20 to 40 C. for at least 3 days, A carbon source, A nitrogen source, An inorganic salt solution, A calcium source at a concentration of 2 to 100 mM andProducing a metabolite in a sterile aqueous production medium comprising: (R)-5,6-dihydro-6-pentyl-2H-pyran-2-one (or (R)-(-)-massoialactone) and / Or (4R, 6R)-4-hydroxy-6-pentyl-tetrahydro-2H-pyran-2-one; and(FIG.
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