Affinity separation matrix for protein purification containing immunoglobulin κ chain variable region

摘要

The present invention provides a peptide excellent in binding ability of an antibody to a κ chain variable region, an affinity separation matrix having the peptide as a ligand, and a method for producing a κ chain variable region-containing protein using the affinity separation matrix. With the goal. Another object of the present invention is to provide DNA encoding the peptide, a vector containing the DNA, and a transformant transformed with the vector. The above problem is solved by using a peptide containing the B5 domain of protein L derived from Peptostreptococcus magnus 312 strain or a part thereof.