Methods for obtaining epigenetic information on cells, methods for determining cell characteristics, methods for determining drug sensitivity or selecting the type of drug or immunotherapeutic method, methods for diagnosing diseases, and self-replicating vectors, assay kits and analysis apparatus

摘要

According to an embodiment, a method for obtaining epigenetic information of cells is provided. This method was obtained by introducing a reporter nucleic acid construct into the nucleus of a test cell, allowing the reporter nucleic acid construct to self-replicate, detecting the presence and / or magnitude of a signal generated in the test cell, Obtaining epigenetic information of the test cell based on the result. Reporter nucleic acid constructs copy the state of modification in a particular sequence on the genome of the test cell onto the corresponding sequence by substitution of functional groups during self-replication in the nucleus of the test cell. It generates a detectable signal depending on the presence of the functional group.