Flavin-binding glucose dehydrogenase with improved substrate specificity

摘要

Provided is a flavin-binding glucose dehydrogenase having a high substrate specificity for D-glucose and a reduced reactivity to D-xylose. One at positions corresponding to positions 42, 83, 174, 367, 385, 386, 391, 460, 461, 467 and 468 of the flavin-binding glucose dehydrogenase derived from the genus Mucor A flavin-binding glucose dehydrogenase having further substitution of amino acid residues. Even when used under conditions where a large amount of enzyme is mounted, such as a glucose sensor, the amount of D-glucose can be measured accurately without being easily affected by the coexistence of D-xylose.