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Method for suppressing the influence of ascorbic acid
Method for suppressing the influence of ascorbic acid
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机译:抑制抗坏血酸影响的方法
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摘要
Provided is a method for suppressing an effect of ascorbic acid on a measurement of glycated hemoglobin in a glycated hemoglobin-containing sample. The method of the present invention is a method for suppressing an effect of ascorbic acid on the measurement of glycated hemoglobin in a glycated hemoglobin-containing sample, comprising reacting the glycated hemoglobin-containing sample with a proteolytic enzyme, reacting a generated glycated peptide with a glycated peptide oxidase, and then measuring a generated hydrogen peroxide, wherein the reaction of the glycated peptide with the glycated peptide oxidase is performed in the presence of a halogen oxide and a substance selected from the group consisting of a substance represented by formula (I), wherein R 1 represents substituted or unsubstituted alkyl and a substance represented by the general formula (II), wherein R 2 represents substituted or unsubstituted alkyl; andrepresents a single bond or a double bond.
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