To provide a high-quality and highly uniform stable supply of nodafuji lectin (WFA) recognizing a biologically important sugar chain marker and a detailed elucidation of the sugar chain recognition activity thereof, It aims to increase the specificity of chain recognition activity. In the present invention, a gene encoding Nodafuji lectin (WFA) was cloned and a method for producing recombinant WFA having sugar chain recognition activity similar to that of natural WFA was developed from a transformed bacterium. Also, by reducing native WFA, a reduced WFA monomer that specifically recognizes a terminal GalNAc residue is prepared, and a cysteine mutation is introduced into recombinant WFA, or a C terminal amino acid deletion causes terminal GalNAc Among sugar chains having residues, a recombinant monomer WFA recognizing an LDN (GaINAc 1,4 GlcNAc) sugar chain, which is important as a diagnostic marker, was produced. Selection drawing None
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