Methods for Sequential Screening with Co-Culture Based Detection of Metagenomic Elements Conferring Heterologous Metabolite Secretion

摘要

The present invention relates to methods associated with metagenomic screening for metabolite induced elements (MIEs) and the subsequent use of the MIEs in screening metagenomic libraries to identify metabolic pathways and pathway components in one or more partial or complete operons. In one aspect the method may be an iterative approach to metagenomic screening which involves substrate and product selection.