MAPPING THE SPATIAL LOCALIZATION OF CELLULAR NUCLEIC ACIDS BY PROXIMITY-DEPENDENT ENZYMATIC TAGGING

摘要

Compositions and methods for mapping the spatial localization of cellular nucleic acids by proximity-dependent enzymatic tagging are described. In particular, proximity-specific biotinylation of nucleic acids is combined with sequencing to identify nucleic acids, including DNA or RNA molecules in proximity to a protein of interest or within or near a particular subcellular compartment in vivo.