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Method for evaluating suitability of duodenal fluid sample as sample for detecting pancreatic fluid-derived components

机译:评价十二指肠液样品作为检测胰液来源成分的适用性的方法

摘要

A method is provided for evaluating the suitability of a duodenal fluid sample collected from an animal as a sample for detecting pancreatic fluid-derived components. The method includes: (a) mixing a duodenal fluid sample with a chymotrypsin-specific substrate and measuring an amount of degradation the chymotrypsin-specific substrate by of the duodenal fluid sample, (b) mixing the duodenal fluid sample with a pepsin-specific substrate and measuring an amount of degradation of the pepsin-specific substrate by the duodenal fluid sample, and (c) evaluating that the duodenal fluid sample is suitable as a sample for detecting pancreatic fluid-derived components if the amount of degradation of the chymotrypsin-specific substrate by the duodenal fluid sample is higher than a prescribed threshold value and the amount of degradation of the pepsin-specific substrate by the duodenal fluid sample is lower than a prescribed threshold value, as being suitable as a sample for detecting pancreatic fluid-derived components.
机译:提供了一种用于评估从动物收集的十二指肠液样品作为检测胰腺液衍生成分的样品的适用性的方法。该方法包括:(a)将十二指肠液样品与胰凝乳蛋白酶特异性底物混合,并测量十二指肠液样品对胰凝乳蛋白酶特异性底物的降解量,(b)将十二指肠液样品与胃蛋白酶特异性底物混合。并测量十二指肠液样品对胃蛋白酶特异性底物的降解量,以及(c)如果胰凝乳蛋白酶特异性的降解量,则评估十二指肠液样品适合作为检测胰液衍生成分的样品十二指肠液样品的底物高于规定的阈值,十二指肠液样品的胃蛋白酶特异性底物的降解量低于规定的阈值,适合作为检测胰液成分的样品。

著录项

  • 公开/公告号US9809837B2

    专利类型

  • 公开/公告日2017-11-07

    原文格式PDF

  • 申请/专利权人 OLYMPUS CORPORATION;

    申请/专利号US201615382996

  • 申请日2016-12-19

  • 分类号C12Q1/37;G01N33/58;

  • 国家 US

  • 入库时间 2022-08-21 13:43:55

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