Oxidation and amination of primary alcohols

摘要

A method including the steps a) providing a primary alcohol of the formula HO—(CH₂)_x—R¹, where R¹ is —OH, —SH, —NH₂ or —COOR²; x is at least 3; and R² is H, alkyl or aryl, b) oxidizing the primary alcohol by contacting it with an NAD(P)⁺-dependent alcohol dehydrogenase, and c) contacting the oxidation product of step a) with a transaminase, where the NAD(P)⁺-alcohol dehydrogenase and/or the transaminase is a recombinant or isolated enzyme. A whole cell catalyst for carrying out the method. The use of such a whole cell catalyst for oxidizing a primary alcohol.